Poster: New Ultra-Sensitive Salmonid Vitellogenin Assay
30th Annual Meeting of the Rocky Mountain Chapter of the Society of Environmental
Toxicology and Chemistry,
Rocky Mountain SETAC, April 13?14, 2017
The TecoMedical Ultra sensitive Salmonid Vitellogenin ELISA shows despite relatively short immune incubation times of total 180 minutes a very high sensitivity. The linear standard range between 0.012 ng/ml and 1 ng/ml with mean intra-assay and inter-assay CVs of 4.4% and 8.0% respectively allow an accurate measurement of vitellogenin above 0.012 ng/ml in different salmonid species.
In non-treated juvenile rainbow trouts no concentration above the detection limit of 0.002 ng/ml was detected. However within 3 days after exposure to E2 a measureable change in vitellogenin concentrations was observed and thus the proof of vitellogenin in salmonid mucus in trace amounts reflects a reliable biomarker for the exposure to exogeneous estrogens.